Journal: Journal of Clinical Medicine

Article Title: Fecal Microbiota Transplantation as New Therapeutic Avenue for Human Diseases

doi: 10.3390/jcm11144119

Figure Lengend Snippet: Stools sample preparation for fecal microbiota transplantation. Fecal stools (100–150 g) dissolved in saline solution (NaCl, 0.9%) are homogenized and larger particles removed by filtration. The fresh fecal sample can be used within less than 6 h for FMT. Alternatively, the fresh fecal preparation is further processed with multiple steps of filtration, cry-protected in glycerol (10%), frozen, and kept at −80 °C for later use. The preparation of FMT capsules involves the addition of freeze-drying protectant glycerol (20%), centrifugation (400× g ), the supernatant is discarded and it is centrifuged again at high speed (10,000× g ); the sediment is incorporated into an enteric-soluble capsule and stored at −80 °C . Alternatively, the material can be lyophilized (vacuum dried) to obtain fecal powder inserted in capsules, and stored at −80 °C for later use. If multiple steps of microfiltration, centrifugation, and suspension are carried out using an automatic system, it is referred to as washed microbiota preparation (WMP). WMP transfer via colonic transendoscopic enteral tubing (TET) is referred to as washed microbiota transplantation (WMT).

Article Snippet: Given the limited alternative methods for symptom relief, Fretheim and co-workers (2020) performed the first FMT with commercially available anaerobic cultivated human intestinal microbiota (ACHIM; developed by ACHIM Biotherapeutics AB), namely, a standardized single donor bacterial mixture, in women with cutaneous systemic sclerosis displaying GI symptoms.

Techniques: Sample Prep, Transplantation Assay, Filtration, Centrifugation

Journal: Journal of Clinical Medicine

Article Title: Fecal Microbiota Transplantation as New Therapeutic Avenue for Human Diseases

doi: 10.3390/jcm11144119

Figure Lengend Snippet: Delivery methods for fecal microbiota transplantation. The delivery methods are classified into upper gastrointestinal routes, including nasogastric/nasoduodenal/nasojejunal tubes and capsules, and lower gastrointestinal routes, including enema colonoscopy and colonic transendoscopic enteral tubing.

Article Snippet: Given the limited alternative methods for symptom relief, Fretheim and co-workers (2020) performed the first FMT with commercially available anaerobic cultivated human intestinal microbiota (ACHIM; developed by ACHIM Biotherapeutics AB), namely, a standardized single donor bacterial mixture, in women with cutaneous systemic sclerosis displaying GI symptoms.

Techniques: Transplantation Assay

Journal: Journal of Clinical Medicine

Article Title: Fecal Microbiota Transplantation as New Therapeutic Avenue for Human Diseases

doi: 10.3390/jcm11144119

Figure Lengend Snippet: Fecal microbiota transplantation in human diseases. Schematic cartoon summarizing fecal microbiota transplantation-based clinical trials in the different human diseases. Refer to the text for details.

Article Snippet: Given the limited alternative methods for symptom relief, Fretheim and co-workers (2020) performed the first FMT with commercially available anaerobic cultivated human intestinal microbiota (ACHIM; developed by ACHIM Biotherapeutics AB), namely, a standardized single donor bacterial mixture, in women with cutaneous systemic sclerosis displaying GI symptoms.

Techniques: Transplantation Assay

Journal: Microorganisms

Article Title: The Effect of Anaerobically Cultivated Human Intestinal Microbiota Compared to Fecal Microbiota Transplantation on Gut Microbiota Profile and Symptoms of Irritable Bowel Syndrome, a Double-Blind Placebo-Controlled Study

doi: 10.3390/microorganisms10091819

Figure Lengend Snippet: Study flow chart for patients with irritable bowel syndrome (IBS) randomized into three subgroups to receive transplantation with donor fecal microbiota transplantation (donor-FMT), anaerobically cultivated human intestinal microbiota (ACHIM) or placebo.

Article Snippet: Anaerobically cultivated human intestinal microbiota (ACHIM, ACHIM Biotherapeutics AB, Vallingby, Stockholm, Sweden) has been suggested for use as an alternative option for donor-FMT, to manipulate gut microbiota [ ].

Techniques: Transplantation Assay

Journal: Microorganisms

Article Title: The Effect of Anaerobically Cultivated Human Intestinal Microbiota Compared to Fecal Microbiota Transplantation on Gut Microbiota Profile and Symptoms of Irritable Bowel Syndrome, a Double-Blind Placebo-Controlled Study

doi: 10.3390/microorganisms10091819

Figure Lengend Snippet: Changes occurring to the Dysbiosis Index before and after receiving transplantation with anaerobically cultivated human intestinal microbiota (ACHIM), donor fecal microbiota transplantation (donor-FMT) or placebo.

Article Snippet: Anaerobically cultivated human intestinal microbiota (ACHIM, ACHIM Biotherapeutics AB, Vallingby, Stockholm, Sweden) has been suggested for use as an alternative option for donor-FMT, to manipulate gut microbiota [ ].

Techniques: Transplantation Assay

Journal: Microorganisms

Article Title: The Effect of Anaerobically Cultivated Human Intestinal Microbiota Compared to Fecal Microbiota Transplantation on Gut Microbiota Profile and Symptoms of Irritable Bowel Syndrome, a Double-Blind Placebo-Controlled Study

doi: 10.3390/microorganisms10091819

Figure Lengend Snippet: Changes occurring to the bacterial strain signals for Actinobacteria spp., Bifidobacteria spp. and Alistipes onderdonkii before and after receiving transplantation with anaerobically cultivated human intestinal microbiota (ACHIM), donor fecal microbiota transplantation (donor-FMT) or placebo. * p < 0.5, ** p < 0.01.

Article Snippet: Anaerobically cultivated human intestinal microbiota (ACHIM, ACHIM Biotherapeutics AB, Vallingby, Stockholm, Sweden) has been suggested for use as an alternative option for donor-FMT, to manipulate gut microbiota [ ].

Techniques: Transplantation Assay

Journal: Microorganisms

Article Title: The Effect of Anaerobically Cultivated Human Intestinal Microbiota Compared to Fecal Microbiota Transplantation on Gut Microbiota Profile and Symptoms of Irritable Bowel Syndrome, a Double-Blind Placebo-Controlled Study

doi: 10.3390/microorganisms10091819

Figure Lengend Snippet: Questionnaire comparison of symptoms before transplantation and after transplantation at each control visit for patients that received anaerobically cultivated human intestinal microbiota.

Article Snippet: Anaerobically cultivated human intestinal microbiota (ACHIM, ACHIM Biotherapeutics AB, Vallingby, Stockholm, Sweden) has been suggested for use as an alternative option for donor-FMT, to manipulate gut microbiota [ ].

Techniques: Comparison, Transplantation Assay, Control

Journal: Microorganisms

Article Title: The Effect of Anaerobically Cultivated Human Intestinal Microbiota Compared to Fecal Microbiota Transplantation on Gut Microbiota Profile and Symptoms of Irritable Bowel Syndrome, a Double-Blind Placebo-Controlled Study

doi: 10.3390/microorganisms10091819

Figure Lengend Snippet: Changes occurring to the IBS-Symptom severity score before and after receiving transplantation with anaerobically cultivated human intestinal microbiota (ACHIM), donor fecal microbiota transplantation (donor-FMT) or placebo.

Article Snippet: Anaerobically cultivated human intestinal microbiota (ACHIM, ACHIM Biotherapeutics AB, Vallingby, Stockholm, Sweden) has been suggested for use as an alternative option for donor-FMT, to manipulate gut microbiota [ ].

Techniques: Transplantation Assay